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Location: Home > Proteins, Peptides & Amino Acids > Recombinant Protein > SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His Recombinant Protein

SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His Recombinant Protein

PH0169M35
Catalog # Availability Size / Price Inquiry
PH0169M35 100ug / $645

SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His Recombinant Protein

Brand

Leading Biology

Catalog Number

PH0169M35

Product Type

Recombinant Protein

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best products. As a result of this work we offer this gene in lyophilized powder format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His Recombinant Protein.

Expression Host

HEK293 Cells

Species

SARS-CoV-2

AA Sequence

(Arg319-Phe541(K417N, E484K, N501Y))

Tags

C-terminus His Tag

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(Arg319-Phe541(K417N, E484K, N501Y))-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85) is 5-40 ng/mL.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Host

HEK293 Cells

UniProt ID

YP_009724390.1

Purity

> 90 % as determined by SDS-PAGE.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Storage & Stability

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Store it under sterile conditions at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Storage Buffer

Lyophilized from sterile PBS, pH 7.4.

Images

Quantity

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