> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Mouse Kitlg/Kit ligand ELISA KitBrand |
Leading Biology | Catalog Number |
EK00292E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00292E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Kit ligand) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Kit ligand The ELISA analytical biochemical technique of the EK00292E kit is based on Kit ligand antibody-Kit ligand antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Kit ligand antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Kit ligand. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Soluble KIT ligand Secreted
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Mouse
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.039 ng/mL
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Assay Range |
0.078-5 ng/ml
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Kitlg
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UniProt ID |
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Function |
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Hematopoietic growth factor KL,Mast cell growth factor,MGF,Steel factor,Stem cell factor,SCF,c-Kit ligand,Kitl,Mgf,Sl,Slf
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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