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General E2/Estradiol ELISA KitBrand |
Leading Biology | Catalog Number |
EK00038E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00038E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Estradiol) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Estradiol The ELISA analytical biochemical technique of the EK00038Ee kit is based on Estradiol antibody-Estradiol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Estradiol antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Estradiol. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
The serum levels of estradiol in males (14 - 55 pg/mL) are roughly comparable to those of postmenopausal women (< 35 pg/mL). Estradiol in vivo is interconvertible with estrone; estradiol to estrone conversion being favored. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs, including the bones.
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
1.33 ng/ml
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Assay Range |
3.12-200 ng/ml
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
E2
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Function |
Estradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3). Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
oestradiol,17β-estradiol
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Images |
Standard curve of the product EK00038E. |
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Specification |
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Quantity |
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