Introduction

For most of soluble protein antigens, we could prepare antibodies by using full-length protein antigens to immune animals, but for those multiple transmembrane proteins which rely on the double lipid membrane structure, the antibody preparation is not that simple. For example, G protein coupled receptor (GPCR) is a typical large, multiple transmembrane protein family. It participates in many pathological signals of many diseases, and it is also a target of nearly half of modern drugs. However, the preparation of GPCR antibody is very difficult. There are mainly two reasons, one is the primary structure of transmembrane protein was divided into several parts on both sides of cell membrane, and to get rid of these transmembrane intervals will cause the loss of protein conformational structures; another reason is that the expression of GPCR is very low, and as a membrane channel which plays a key role in signal transduction and expression, overexpression of exogenous human GPCR will leading to cell signal disorder, and extremely easy to cause cell death, so it is very difficult for us to get even the minimum amount of protein required for protein measurement.

Leading Biology has years of practical experience, accumulated a number of multiple transmembrane protein antibody preparation methods, including the design of the classical soluble region protein and recombinant protein immunization scheme, also the whole cell immune antibody preparation method designed by our technicians to the minimize the risk, obtain the best quality antibodies.

Service package

Classic case 

(Twice Transmembrane antibody service)

Customer Provide

Protein information

Process

1. protein structural analysis, recombinant protein design and codon optimization.

2. Gene synthesis, vector construction and protein expression.

3. Feeding and immunization of 2 rabbits/antigen (3 immunizations, 3 weeks)

4. Blood collection, ELISA test ( parallel with immunization)

5. Purification, detection and delivery

Products

(each antigen, 2 rabbits)

1. 3-5mL negative serum.

2. 15-20mL positive serum, ELISA titer was above 1:60,000.

3. Complete lab report, remaining antigens.

Preparation time: About 3-4 months

Successful Result

Publication: Nat Cell Biol        

IF:7.73

Title: Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells.

Name: Anti-fundc1 antibody

Species: Human

Type: Rabbit polyclonal antibody

F1. HeLa cells were transfected with plasmids encoding FUNDC1–Myc for the indicated times and the mitochondrial (TIM23, TOM20, VDAC1), endoplasmic reticulum (calnexin) and Golgi marker (GM130) proteins were detected by western blotting.

Related Services

Anti-Membrane Protein Antibody Discovery

Anti-autologous Antigen Antibody Production Service

Antibody Production by Liposome Immunization

Anti-hapten Antibody Production Service

Membrane Protein Antibody Production

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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By Phone: 1-661-524(LBI)-0262 (USA)