Introduction

Leading Biology, with over 5 years of experience in developing antibodies, is a leading global provider of custom antibody production services. Our facility generates high-affinity custom antibodies with industry-leading titer guarantees and comprehensive antibody production packages that simplify the ordering process and present cost-effective methods to isolate epitope-specific antibodies. 

The efficiency of the antibody depends largely on three aspects: the characterization of antigen, the state of the immune animals and the immunization method. Leading Biology constructs a standardized biological environment for the immune animals, at the same time has high quality breeding and an experimental team to guaranteed these aspects.

More than half of the antibody preparation failure is caused by the property of the antigen itself. For example, for membrane protein antigens, small molecule antigens or self-antigens, because of their special structures (such as hidden structures), weak immunogenicity (such as small space volume), or because of autoimmune tolerance problem, it is difficult to produce effective immune responses. So the professional teams of Leading Biology always make sure that all services of antibody preparation should fully understand the properties of antigen, according to their specific properties to design scheme of antibody preparation, and do reasonable construction on special antigen to achieve the purpose of the experiment.

Service details

Antigen Forms
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.

Antigen Quantity
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.

Antigen Concentration
Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.

Considerations for Gel Bands
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands, in particular, it is important to maintain a higher concentration of the protein as outlined above.

Shipping Antigens to Leading Biology

Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form. 

Related Services

Anti-Membrane Protein Antibody Discovery

Anti-autologous Antigen Antibody Production Service

Antibody Production by Liposome Immunization

Anti-hapten Antibody Production Service

Membrane Protein Antibody Production

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry-leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)