• Account
  • Account
    Inquiries
    0

    Inquired items

    0items total Subtotal:$ 0
  • Cart
    Cart

    Inquired items

    items total Subtotal:$ 0

All Departments

Location: Home > Custom Services > Antibody Discovery Services > Interference Function and Anti-idiotype Antibody Service

Interference Function and Anti-idiotype Antibody Service

Date: 2018-08-27 Author: Leading Biology Click: 559

Introduction

Although a wide variety of antigens in the immune animal could provide a wide variety of antibodies, even the same antigen processed by immune cells could present different antigen information to B lymphocytes, so the pairing relationship between antigens and antibodies is mainly multiple to one, but in converse, for a specific antibody, there is only one antigen or antigen information could be identified. Among the hundreds of amino acids that constitute an antibody, most of the sequences are highly conserved in the same antibody subtype family. Only a few of them which closely related to antigen binding are different, and these amino acid sequences are unique sequences identifying specific antigen.


In many medical or biological studies, it is often needed to do qualitative and quantitative analyses for the specific antibodies induced by antigens (such as a drug), in order to get some indicator information (such as some pharmacokinetics indicators). If there is no specific anti-antibodies to identify specific antibodies, the immunoglobulin family derived from the target antibody will cause great interference to the experiment, even submerged the meaningful experimental data in the background. And as for some antibody which itself is an antigen (such as antibody drug), it is often difficult to effectively trigger the immune response, and to distinguish these antibodies and antibody form the body itself, to achieve in situ or real-time detection, we also need specific anti-antibodies which could identify specific antibody.

Fig. 1 For an antibody, the specific binding site of antigen is also the core location for the antibody identification, the epitope of a specific antibody is usually located in this region.


On the one hand, the specificity of the antibody ensures the specificity of the recognition, on the other hand, these antibodies often accompanied by repression function, the target antigen (i.e. the antibody which be identified, referred as TA) combination could block the original antigen (i.e. the antigen which identified by TA, also recognized by primary antigen, PA) binding.


Due to the unique function mentioned above, these specific anti-antibodies are often used in the study of pharmacokinetics (PK).


Fig. 2 The difference between three binding forms of specific anti-antibody (B, C, D) and normal secondary antibody (A)

A. The normal combination of the secondary antibody and primary antibody only have species specificity, but no specificity of antibody recognition. It can be used in the PK study in the form of antigen capture.

B. Preparation of antigen-antibody complex antibody can be used in PK study of conjugated drugs, this antibody only recognizes antibodies which in binding state, thus has high specificity for drug binding recognition, but the preparation is difficult, and it also have high requirement of the TAb binding force and  conformation stability.

C. The most commonly binding form used in the PK study of antibody drugs (AD) or anti-drug antibodies (ADA).

D. Binding specific anti-antibody preparation is based on C, by using capture-targeting-reporting conjugation method, detect target antibody directly in the original habitat, according to the experimental purpose and PK experimental design, there are different experimental requirements for two antibodies specification.


Leading Biology established a complete set of experiments, quality control and verification method for project management, at the same time integrates the enterprise resources for the further application of downstream antibody test, such as full service of pharmacokinetic/pharmacodynamic study (PK/PD).


Service Package

Service Items

Time

(months)

Start point

Purified protein

Expression plasmid

Prokaryotic proteins synthesis de novo

Eukaryotic proteins synthesis de novo

Proteins preparation

cDNA synthesis

1-2

 

 

 

 

Need further inquiry

Vector construction

 

 

 

 

Protein expression & purification

 

 

 

Immunization

Immune 5 mice

1.5

ELISA titer check

Cell Fusion & Screening

1.5

IgG protein family negative screening (subcloning)

Ascites production

(Inject 3 mice)

0.5

Function suppression or Non-suppression screening

1

Large scale paired antibody screening

1.5

 

 

 

 

Ascites production

(Inject 5 mice)

1

Purification and detection

0.5

Total lead time(months)

4-6

6-8

4-6

6-8

5-7

6.5-8.5

>6

>8

Recommend package

Target Region anti-Antibody Service  package

 Special project plan, comprehensive risk assessment

 Immunization and Fusion of 5 mice

Large scale pipelined screening, save time

 Ideal for antibody drug R&D projects

 High special anti-antibody

Note:

1). Screening functional repression or non-repression specific anti-antibodies according to the requirements of the customer.

2). Determine the project according to the customer's own needs, we could provide paired double repression antibodies, paired single repression antibodies, or heterologous double repression antibodies. We will charge 60% of the total price of the project in advance.

3). For different experimental purposes, there are different combinations of plans, and the prices above are just list price, the actual price will based on the actual needs of the project.


Related Services

IHC-Positive Hybridoma Screening

Capture\Report Paired Antibody Service

Anti-isotype Antibody Production

Custom Antibody Services for Post-translational Modification

Ligand Memetics Agonistic Antibody Discovery Service

Interference Function/Anti-idiotype Antibody Service


Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * captcha
No Headline Click Author Date
1 Mouse Monoclonal Antibody preparation by phage display 261 Leading Biology 2019-11-20
2 Rabbit Monoclonal Antibody preparation by phage display 255 Leading Biology 2019-11-20
3 Antibody Labeling Services 610 Leading Biology 2019-11-05
4 Custom Monospecific Antibody Production 415 Leading Biology 2019-11-04
5 Custom Peptide Antibody Production 350 Leading Biology 2019-11-04
6 Custom Phosphospecific Antibody Production 532 Leading Biology 2019-11-04