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Location: Home > Custom Services > Antibody Discovery Services > Anti-isotype Antibody Production

Anti-isotype Antibody Production

Date: 2018-08-27 Author: Leading Biology Click: 785

Introdution

The immune process of animals is done by the participation of a variety of organs and cells, so, even for the same antigen immunization, there is a great difference between the antibody produced by the same antigen in the same immune cycle. Taking people and mice as an example, there are five subtypes of antibodies in the body, they are: IgG, IgA, IgM, IgE and IgD, of which IgG is divided into four subtypes according to the location of the disulfide bond, which also called homotypes. In some immunological tests, in order to observe the stage of the immune response of the tested animal, it is often needed to use the subtype of the main antibody produced by the animal as a clue. Capture or mark a specific subtype of antibody,  at the same time have no specific identification of antibodies against specific antigens, this is the so-called anti specific subtype antibody, it is the basic material for the identification of antibody subtypes.


Service Package

Service Items

Time (months)

Start point

Purified single isotype antibodies

Mixed antibodies (serum)

Prokaryotic proteins synthesis de novo

Eukaryotic proteins synthesis de novo

Antigens preparation

cDNA synthesis

1-2

 

 

 

 

Need further inquiry

Vector construction

 

 

 

 

Isolating single isotype antibodies and purification

 

 

 

 

 

 

 

Protein expression & purification

 

 

 

 

Enzyme treatment and recovering

 

 

 

 

Immunization

Immune 5 mice or 2 rabbits

1.5

ELISA titer test

Cell Fusion & Screening

1.5

 

 

 

 

Same isotype Immunoglobulin protein  positive screening (subcloning)

 

 

 

 

Ascites production

(Inject 2 mice)

0.5

 

 

 

 

Different isotype Immunoglobulin protein negative screeni

0.5

Ascites production

(Inject 5 mice)

1

 

 

 

 

Purification and detection

0.5

Total lead time(months)

4-6

6-8

4-6

6-8

5-7

6.5-8.5

>6

>8


Related Services

IHC-Positive Hybridoma Screening

Capture\Report Paired Antibody Service

Anti-isotype Antibody Production

Custom Antibody Services for Post-translational Modification

Ligand Memetics Agonistic Antibody Discovery Service

Interference Function/Anti-idiotype Antibody Service


Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:


• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)

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