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Location: Home > Custom Services > Antibody Discovery Services > Ligand Memetics Agonistic Antibody Discovery Service

Ligand Memetics Agonistic Antibody Discovery Service

Date: 2018-08-27 Author: Leading Biology Click: 382

Introduction

Cell signal transduction is the core content of cell functional omics research. And the binding of receptors and ligands to activate the cell signaling is the starting step of many activation or inhibitory signaling pathways. By preparing the antibodies related to cell surface signal receptors to simulate the combination of receptors and ligands on the original body cell surface, thus initiating cell signaling pathways, such antibodies called ligand mimic activator antibody. The most classic example is the antibody drugs used in tumor targeting therapy, at present, there are at least 11 types of monoclonal antibody drugs have been found to be functional by receptor activation.


At present, in addition to the conventional rabbit/rat polyclonal antibody, and monoclonal antibody hybridoma cell preparation and screening program, based on phage display technology, Leading Biology is also able to provide the service of discovery of new receptor-activated antibodies, we not only have a common natural antibody library, but also could construct a phage library based on the advanced prokaryotic or eukaryotic expression technology and the information of specific antigens, then doing the specific antibody screening for receptor recombinant extracellular domain (ECD). The single-chain antibody obtained through the initial screening of phage ELISA, could further do some functional screening based on cell experiments. We have separated TNF receptor superfamily, CD28,CD40,PD1,CD127, etc.


Service package

Service Items

Time(weeks)

Immunized library

Naive library

Hybridoma Screening

Peptide design & synthesis

Peptide design

-2

Free, Optional

Peptide synthesis

 

 

 

 

Conjugation

 

 

 

 

Proteins preparation

cDNA synthesis

6-8

 

 

 

 

Need further inquiry

Vector construction

 

 

 

 

Protein expression & purification

 

 

 

 

Immunization

Immune

animals

-8

 

 

ELISA test

 

 

Cell Fusion & Screening

1.5

 

 

 

 

Subcloning

1

 

 

 

 

Ascites production

(Inject 2 mice)

1

 

 

 

 

cDNA preparation

Prepare cDNA library by RT-PCR

-1

 

 

 

 

cDNA library QC check

 

 

 

 

Fab library construction

Obtained VH and VL genes

10-15

 

 

 

 

Primary antibody library construction

 

 

 

 

Fab library construction

 

 

 

 

Phage screening

Phage particles package

10-15

 

 

Magnetic beads affinity screening

 

 

Recover positive clones and DNA sequencing

 

 

Antibody expression

Synthetic of target phage gene and Fc gene

-5

 

 

Eukaryotic system expression plasmids construction

 

 

Eukaryotic system expression

 

 

Stably transfected cell line construction

 

 

 

 

 

 

Purification and detection

Lable tag affinity purification

-1

 

 

Affinity purification

 

 

 

 

Antigen specific affinity purification

 

 

Total lead time(months)

7-9


Related Services

IHC-Positive Hybridoma Screening

Capture\Report Paired Antibody Service

Anti-isotype Antibody Production

Custom Antibody Services for Post-translational Modification

Ligand Memetics Agonistic Antibody Discovery Service

Interference Function/Anti-idiotype Antibody Service


Why Leading Biology

With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:

• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.

• Our economies of scale allow us to pass cost savings to you and help maximize your budget.

• 100% of services are transparent throughout.

• Antibodies from our facility have been cited in many published research papers.

• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.

• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.

• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)

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