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Ligand Memetics Agonistic Antibody Discovery ServiceIntroduction
Cell signal transduction is the core content of cell functional omics research. And the binding of receptors and ligands to activate the cell signaling is the starting step of many activation or inhibitory signaling pathways. By preparing the antibodies related to cell surface signal receptors to simulate the combination of receptors and ligands on the original body cell surface, thus initiating cell signaling pathways, such antibodies called ligand mimic activator antibody. The most classic example is the antibody drugs used in tumor targeting therapy, at present, there are at least 11 types of monoclonal antibody drugs have been found to be functional by receptor activation.
At present, in addition to the conventional rabbit/rat polyclonal antibody, and monoclonal antibody hybridoma cell preparation and screening program, based on phage display technology, Leading Biology is also able to provide the service of discovery of new receptor-activated antibodies, we not only have a common natural antibody library, but also could construct a phage library based on the advanced prokaryotic or eukaryotic expression technology and the information of specific antigens, then doing the specific antibody screening for receptor recombinant extracellular domain (ECD). The single-chain antibody obtained through the initial screening of phage ELISA, could further do some functional screening based on cell experiments. We have separated TNF receptor superfamily, CD28,CD40,PD1,CD127, etc.
Service package
Service Items |
Time(weeks) |
Immunized library |
Naive library |
Hybridoma Screening |
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Peptide design & synthesis |
Peptide design |
-2 |
Free, Optional |
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Peptide synthesis |
√ |
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√ |
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Conjugation |
√ |
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|
√ |
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Proteins preparation |
cDNA synthesis |
6-8 |
|
√ |
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Need further inquiry |
Vector construction |
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√ |
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√ |
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Protein expression & purification |
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√ |
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√ |
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Immunization |
Immune animals |
-8 |
√ |
√ |
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√ |
√ |
ELISA test |
√ |
√ |
|
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√ |
√ |
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Cell Fusion & Screening |
1.5 |
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√ |
√ |
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Subcloning |
1 |
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|
√ |
√ |
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Ascites production (Inject 2 mice) |
1 |
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√ |
√ |
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cDNA preparation |
Prepare cDNA library by RT-PCR |
-1 |
√ |
√ |
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cDNA library QC check |
√ |
√ |
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Fab library construction |
Obtained VH and VL genes |
10-15 |
√ |
√ |
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Primary antibody library construction |
√ |
√ |
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Fab library construction |
√ |
√ |
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Phage screening |
Phage particles package |
10-15 |
√ |
√ |
√ |
√ |
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Magnetic beads affinity screening |
√ |
√ |
√ |
√ |
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Recover positive clones and DNA sequencing |
√ |
√ |
√ |
√ |
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Antibody expression |
Synthetic of target phage gene and Fc gene |
-5 |
√ |
√ |
√ |
√ |
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Eukaryotic system expression plasmids construction |
√ |
√ |
√ |
√ |
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Eukaryotic system expression |
√ |
√ |
√ |
√ |
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Stably transfected cell line construction |
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Purification and detection |
Lable tag affinity purification |
-1 |
√ |
√ |
√ |
√ |
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Affinity purification |
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√ |
√ |
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Antigen specific affinity purification |
√ |
√ |
√ |
√ |
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Total lead time(months) |
7-9 |
Related Services
IHC-Positive Hybridoma Screening
Capture\Report Paired Antibody Service
Anti-isotype Antibody Production
Custom Antibody Services for Post-translational Modification
Why Leading Biology
With over 5 years of experience in custom antibodies, we offer numerous benefits for developing your custom antibody:
Contact Information• Our industry leading titer guarantees of 1:50,000 eliminate the risk of not obtaining antibodies against peptide antigens.
• Our economies of scale allow us to pass cost savings to you and help maximize your budget.
• 100% of services are transparent throughout.
• Antibodies from our facility have been cited in many published research papers.
• By outsourcing antibody production and characterization needs to us, you can spend more time focused on your research.
• Our ideal location in California ensures that animals benefit from outdoor facilities and mild year-round temperatures.
• Confidentiality is a top priority for all projects. Antibodies that we manufacture belong to you and will not be commercialized.
Please obtain a quote before ordering, and refer to the quote number when you place an order.
Orders are typically confirmed within 12 hours.
Have a Question? Email us info@leadingbiology.com
Order Products: Order Related Products
By Phone: 1-661-524(LBI)-0262 (USA)
No | Headline | Click | Author | Date |
1 | Antibody Production | 2540 | Leading Biology | 2020-01-16 |
2 | Mouse Monoclonal Antibody preparation by phage display | 1315 | Leading Biology | 2019-11-20 |
3 | Rabbit Monoclonal Antibody preparation by phage display | 1695 | Leading Biology | 2019-11-20 |
4 | Antibody Labeling Services | 5730 | Leading Biology | 2019-11-05 |
5 | Custom Monospecific Antibody Production | 1985 | Leading Biology | 2019-11-04 |
6 | Custom Peptide Antibody Production | 6602 | Leading Biology | 2019-11-04 |