> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human Chromogranin A (CHGA) ELISA KitBrand |
Leading Biology | Catalog Number |
EK00844E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
This ELISA Kit uses the double-antigen sandwich method to determine the level of human chromogranin A (CHGA)in the specimen. The ELISA analytical biochemical technique of the kit is based on CHGA antigen-CHGA antibody interactions (immunosorbency) and an HRP colorimetric detection system to detect CHGA targets in samples. CHGA are added to the antibody-coated microwells, and then combine with the HRP-labeled antigen to form an antibody-antigen-Enzyme-labeled antibody complex, after thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color intensity is positively correlated with CHGA in the sample. Absorbance (OD value) is measured at a wavelength of 450nm with a microplate reader, and the concentration of human CHGA in the sample can be calculated from the standard curve.
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Assay Type |
Sandwich
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Reactivity |
Human
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Sample Type |
Serum, Plasma, Other human body fluids & secretions
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Assay Range |
20ng/mL g/L – 480ng/mL
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Kit Components |
Assay Plate, Standard, Standard diluent, Sample diluent, Enzyme conjugate, Detection reagent A & B, Wash buffer, Stop solution, Protocol, Quality control certificate
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Storage & Stability |
Store at 2-8 °C
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Product Description |
Results of a typical standard curve are provided with the product for demonstration only and should not be used to obtain test results. A standard curve must be run for each set of samples assayed. After receiving the kit, please check the intactness of outer packaging, check whether the reagent bottles are broken, leaking or the amount of liquid is significantly reduced, etc. If the diluent is polluted such as flocculent bacterial colonies; the substrate is not transparent and the microtiter plate is leaking, etc; please contact the distributor. In rare cases, crystals may appear in the substrate. Normally the crystals will dissolve at room temperature, this will not affect the testing results. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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