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Functional Rab6-GTP Antibody, mAb (recombinant)

APR16045G Product Datasheet Protocol

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APR16045G		100 μg / $545

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Leading Biology Inc.

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Functional Rab6-GTP Antibody, mAb (recombinant)

Brand	Leading Biology	Catalog Number	APR16045G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Functional Rab6-GTP antibody, mAb (recombinant).
Molecular Weight	23593 Da
Cellular Localization	Antigen Cellular Localization: Golgi apparatus. Golgi apparatus membrane; Lipid- anchor
Host	Purified From?HEK 293 Cell?culture Supernatant.
Species Reactivity	Human, Mouse, Drosophila
Target	Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6?GDP.
Clone	AA2
Isotype	Human IgG2λ
Symbol	RAB6
GeneID	5870
UniProt ID	P20340
Function	Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity.
Summary	Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
Form	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	ICC
Synonyms	Ras-related Protein Rab-6
Images	Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1ug /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr Moutel, Dr Franck Perez Lab, Curie Institute, Paris.
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