Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Functional Rab1-GTP Antibody, mAb (recombinant)

Functional Rab1-GTP Antibody, mAb (recombinant)

APR16044G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
APR16044G		100 μg / $545

Bulk Orders Inquiry

1 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

Functional Rab1-GTP Antibody, mAb (recombinant)

Brand	Leading Biology	Catalog Number	APR16044G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Functional Rab1-GTP antibody, mAb (recombinant).
Molecular Weight	22678 Da
Cellular Localization	Antigen Cellular Localization: Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm, cytosol. Membrane. Melanosome. Note=Alternates between membrane-associated and cytosolic forms
Host	Purified From?HEK 293 Cell?culture Supernatant.
Species Reactivity	Human, Mouse, Rat, Dog
Target	Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.
Clone	ROF7
Isotype	Human IgG2λ
Symbol	RAB1
GeneID	5861
UniProt ID	P62820
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
Summary	Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking.
Form	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	ICC, IP
Synonyms	Ras-related Protein Rab-1
Images	Rab1-GTP is detected by immunocytochemistry using anti-Rab1-GTP, mAb (ROF7). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab1-GTP, mAb (ROF7)(1mg /ml ) in PBS-BSA-Saponin). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: Functional Rab6-GTP Antibody, mAb (recom... Next Product: FGF23 (Fibroblast Growth Factor 23) Anti...

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APG02467G	CCK4 / PTK7 Antibody (clone 4F9)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
2	Leading Biology	AMM04683G	GALT Antibody (clone 4C11)	50 μg	Monoclonal Antibodies				$545.00	Add Ask
3	Leading Biology	AMM01402G	Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
4	Leading Biology	APR08280G	LTA4H / LTA4 Antibody (clone 9G8)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
5	Leading Biology	AMM00172G	CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
6	Leading Biology	AMM05750G	CEBPA Antibody	100 μl	Monoclonal Antibodies				$545.00	Add Ask