• Account
  • Account
    Inquiries
    0

    Inquired items

    0 items total Subtotal:$ 0
  • 会员中心
    Cart

    Added items

    0items total Subtotal:$ 0

All Departments

CYK18 Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR15652G 100 μl / $495

CYK18 Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR15652G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CYK18 antibody (C-term).

Molecular Weight

48058 Da

Cellular Localization

Antigen Cellular Localization: Cytoplasm, perinuclear region. Nucleus, nucleolus

Host

Mouse

Species Reactivity

Human

Target

This CYK18 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 400-430 region of human CYK18.

Clone

695CT17.2.6

Isotype

IgG3

Symbol

CYK18

GeneID

UniProt ID

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Summary

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Form

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

IF, WB, E

Dilution

IF~~1:25 WB~~1:1000

Synonyms

Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18

Images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AM2178B at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AM2178B at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

All lanes : Anti-CYK18 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCI-H460 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Specification

Quantity

Related Products

Select Brand Catalog No. Product Name Pack Size Type Field of Research Specification Quantity Price(USD)
1 Leading Biology APG02467G CCK4 / PTK7 Antibody (clone 4F9) 50 μl Monoclonal Antibodies
$495.00 Add Ask
2 Leading Biology AMM04683G GALT Antibody (clone 4C11) 50 μg Monoclonal Antibodies
$545.00 Add Ask
3 Leading Biology AMM01402G Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
4 Leading Biology APR08280G LTA4H / LTA4 Antibody (clone 9G8) 50 μl Monoclonal Antibodies
$495.00 Add Ask
5 Leading Biology AMM00172G CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody - With BSA and Azide 50 ug Monoclonal Antibodies
$395.00 Add Ask
6 Leading Biology AMM05750G CEBPA Antibody 100 μl Monoclonal Antibodies
$545.00 Add Ask

Contact Us

This item has successfully been added to your cart!

You have 0 item in your cart

Your inquiry has successfully been added to your list!

You have 0 item in your inquiry list