Location: Home > Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > SOX2 Antibody (Ascites)

SOX2 Antibody (Ascites)

APR14253G Product Datasheet Protocol

Catalog #	Availability	Size / Price	Inquiry
APR14253G		0.1 ml / $545

Bulk Orders Inquiry

3 Images

Contact Information

Leading Biology Inc.

tel:1-661-524(LBI)-0262
Info@leadingbiology.com

Show All Distributors

SOX2 Antibody (Ascites)

Brand	Leading Biology	Catalog Number	APR14253G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SOX2 antibody (Ascites).
Molecular Weight	34310 Da
Cellular Localization	Antigen Cellular Localization: Nucleus.
Host	Mouse
Species Reactivity	Human
Target	Human SOX2 recombinant protein is used to produce this monoclonal antibody.
Clone	57CT23.3.4
Isotype	IgG1
GeneID	6657
UniProt ID	P48431
Function	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Summary	This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Form	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	IF, WB, FC, E
Dilution	IF~~1:200 WB~~1:200~2000 FC~~1:50~100
Synonyms	Transcription factor SOX-2, SOX2
Images	Fluorescent confocal image of an SY5Y cell stained with APR14253G SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with APR14253G SOX2 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 3 min). SOX2 immunoreactivity is localized exclusively to the nucleus. Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat. APR14253G) by human SOX2 recombinant protein cell line lysate (35μg/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab. Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
Specification	Product Datasheet Protocol
Quantity

Add to cart Inquiry

Previous Product: THBS1 Antibody (Center) Next Product: FGF23 (Fibroblast Growth Factor 23) Anti...

Best Sellers

Related Products

Select	Brand	Catalog No.	Product Name	Pack Size	Type	Field of Research	Specification	Quantity	Price(USD)
1	Leading Biology	APG02467G	CCK4 / PTK7 Antibody (clone 4F9)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
2	Leading Biology	AMM04683G	GALT Antibody (clone 4C11)	50 μg	Monoclonal Antibodies				$545.00	Add Ask
3	Leading Biology	AMM01402G	Vimentin (Mesenchymal Cell Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
4	Leading Biology	APR08280G	LTA4H / LTA4 Antibody (clone 9G8)	50 μl	Monoclonal Antibodies				$495.00	Add Ask
5	Leading Biology	AMM00172G	CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody - With BSA and Azide	50 ug	Monoclonal Antibodies				$395.00	Add Ask
6	Leading Biology	AMM05750G	CEBPA Antibody	100 μl	Monoclonal Antibodies				$545.00	Add Ask