> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Ubiquitin Antibody (C-term)Brand |
Leading Biology | Catalog Number |
APR13901G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Ubiquitin antibody (C-term).
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Host |
Rabbit
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Species Reactivity |
Human, Mouse, Rat
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Target |
A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.
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Clone |
EP296Y
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UniProt ID |
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Summary |
Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).
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Form |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IHC
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Dilution |
WB~~1:1000
IHC~~1:250~500
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Synonyms |
UBC, UBCEP2, Ubiquitin
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Images |
A. Western blot analysis on Hela cell lysate using anti-Ubiquitin (C-term) RabMAb (Cat. APR13901G), dilution 1:2,000.
B. Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using anti-Ubiquitin (C-term) RabMAb (Cat. APR13901G). |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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