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Ubiquitin Antibody (C-term)

Catalog # Availability Size / Price Inquiry
APR13901G 100 μl / $545

Ubiquitin Antibody (C-term)

Brand

Leading Biology

Catalog Number

APR13901G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Ubiquitin antibody (C-term).

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Target

A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.

Clone

EP296Y

UniProt ID

Summary

Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).

Form

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC

Dilution

WB~~1:1000 IHC~~1:250~500

Synonyms

UBC, UBCEP2, Ubiquitin

Images

A. Western blot analysis on Hela cell lysate using anti-Ubiquitin (C-term) RabMAb (Cat. APR13901G), dilution 1:2,000.

B. Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using anti-Ubiquitin (C-term) RabMAb (Cat. APR13901G).

Specification

Quantity

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