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NDUFAF1 Antibody

Catalog # Availability Size / Price Inquiry
APR08657G 100 μl / $545

NDUFAF1 Antibody

Brand

Leading Biology

Catalog Number

APR08657G

Product Type

Monoclonal Antibodies

Field of Research

Product Overview

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NDUFAF1 antibody.

Molecular Weight

37764 Da

Cellular Localization

Antigen Cellular Localization: Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Target

A synthetic peptide corresponding to residues at the C terminus of human NDUFAF1 was used as an immunogen.

Clone

EPR2796

Symbol

CIA30

GeneID

UniProt ID

Function

Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).

Summary

NDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2).

Form

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Applications

WB, IHC, IF

Dilution

WB~~1:1000~2000 IHC~~1:100~250

Synonyms

Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, NDUFAF1, CIA30

Images

A. Western blot analysis on (A) HeLa, (B) Jurkat and (C) human heart lysates using anti-NDUFAF1 RabMAb (Cat. APR08657G).

B. Immunohistochemical analysis of paraffin-embedded human liver using anti-NDUFAF1 RabMAb (Cat. APR08657G).

C. Immunofluorescent staining of HeLa cells using anti-NDUFAF1 RabMAb (Cat. APR08657G).

Specification

Quantity

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