> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Vimentin AntibodyBrand |
Leading Biology | Catalog Number |
AMM02593G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Vimentin Antibody.
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Molecular Weight |
50 KDa
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Host |
Mouse
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Species Reactivity |
Bovine, Human, Mouse, Rat
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Target |
Recombinant human vimentin purified from E. coli.
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Clone |
2D1
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Isotype |
IgG2a
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GeneID |
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UniProt ID |
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Summary |
Vimentin is the major protein subunit of the 10nm or intermediatefilaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developingneuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involvedin lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers arefrequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes),desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies tovimentin are useful in studies of stem cells andgenerally to reveal the filamentous cytoskeleton.Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness(Zhao et al. 2008).
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, IF
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Dilution |
WB~~ 1:1000
IF~~ 1:500
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Images |
Western blot of HeLa cells showing specific immunolabeling of the ~50k vimentin protein.
Mixed neuron/glial cultures stained with anti-vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments. |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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