> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)Brand |
Leading Biology | Catalog Number |
AMP00010G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant).
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Molecular Weight |
223044 Da
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm, myofibril. Note=Thick filaments of the myofibrils
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Host |
Purified From?HEK 293 Cell?culture Supernatant.
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Species Reactivity |
Human, Mouse, Rat, Drosophila
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Target |
Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
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Clone |
SF9
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Isotype |
Human IgG2λ
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Symbol |
MYHSA2
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GeneID |
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UniProt ID |
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Function |
Muscle contraction. Required for cytoskeleton organization (By similarity).
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Summary |
anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
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Form |
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, ICC, E
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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