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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Chicken C-Peptide/C-Peptide ELISA Kit

Chicken C-Peptide/C-Peptide ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00828E 96T / $745

Chicken C-Peptide/C-Peptide ELISA Kit

Brand

Leading Biology

Catalog Number

EK00828E

Product Type

ELISA Kit

Field of Research

Metabolism

Product Overview

EK00828E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-Peptide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-Peptide The ELISA analytical biochemical technique of the EK00828E kit is based on C-Peptide antibody-C-Peptide antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-Peptide antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-Peptide. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Cellular effects of C-peptide: C-peptide has been shown to bind to the surface of a number of cell types such as neuronal, endothelial, fibroblast and renal tubular, at nanomolar concentrations to a receptor that is likely G-protein-coupled. The signal activates Ca2+-dependent intracellular signaling pathways such as MAPK, PLCγ, and PKC, leading to upregulation of a range of transcription factors as well as eNOS and Na+K+ATPase activities.

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Chicken

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

0.087 ng/mL

Assay Range

0.312-20 ng/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

C-Peptide

Function

C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Images

Standard curve of the product EK00828E.

Specification

Quantity

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