> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human INPP5D/Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00807E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00807E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1 The ELISA analytical biochemical technique of the EK00807E kit is based on Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1 antibody-Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cytoplasm Cell membrane Peripheral membrane protein Membrane raft Cytoplasm Cytoskeleton Membrane Peripheral membrane protein Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Translocates from the cytoplasm to membrane ruffles in a FCGR3/CD16-dependent manner. Colocalizes with FC-gamma-RIIB receptor (FCGR2B) or FCGR3/CD16 at membrane ruffles. Tyrosine phosphorylation may also participate in membrane localization.
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.15 ng/mL
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Assay Range |
0.312-20 ng/ml
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
INPP5D
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MIM |
601582
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UniProt ID |
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Function |
Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:8723348, PubMed:10764818, PubMed:8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9108392, PubMed:10764818, PubMed:8769125). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed:16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
SHIP,SHIP1,Inositol polyphosphate-5-phosphatase D,Inositol polyphosphate-5-phosphatase of 145 kDa,SIP-145,Phosphatidylinositol 4,5-bisphosphate 5-phosphatase,SH2 domain-containing inositol 5'-phosphatase 1,SH2 domain-containing inositol phosphatase 1,p150Ship,hp51CN
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Images |
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Specification |
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Quantity |
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2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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