> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human SRSF1/Serine/arginine-rich splicing factor 1 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00802E |
Product Type |
ELISA Kit | Field of Research |
Epigenetics |
Product Overview |
EK00802E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Serine/arginine-rich splicing factor 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Serine/arginine-rich splicing factor 1 The ELISA analytical biochemical technique of the EK00802E kit is based on Serine/arginine-rich splicing factor 1 antibody-Serine/arginine-rich splicing factor 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Serine/arginine-rich splicing factor 1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Serine/arginine-rich splicing factor 1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cytoplasm Nucleus speckle In nuclear speckles. Shuttles between the nucleus and the cytoplasm.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.12 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
SRSF1
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MIM |
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UniProt ID |
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Function |
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Alternative-splicing factor 1,ASF-1,Splicing factor, arginine/serine-rich 1,pre-mRNA-splicing factor SF2, P33 subunit,ASF,SF2,SF2P33,SFRS1,OK/SW-cl.3
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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