> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > General Oxtc/Oxytocin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00794E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00794E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Oxytocin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Oxytocin The ELISA analytical biochemical technique of the EK00794Ee kit is based on Oxytocin antibody-Oxytocin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Oxytocin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Oxytocin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Oxytocin is a peptide of nine amino acids (a nonapeptide). Its systematic name is cysteine-tyrosine-isoleucine-glutamine-asparagine-cysteine-proline-leucine-glycine-amine (cys – tyr – ile – gln – asn – cys – pro – leu – gly - NH2, or CYIQNCPLG-NH2). The cysteine residues form a disulfide bond. Oxytocin has a molecular mass of 1007 daltons. One international unit (IU) of oxytocin is the equivalent of about 2 micrograms of pure peptide.
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
9.4 pg/mL
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Assay Range |
31.2-2000 pg/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Oxtc
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Function |
Oxytocin is a mammalian hormone that acts primarily as a neuromodulator in the brain.
Oxytocin is best known for its roles in sexual reproduction, in particular during and after childbirth. It is released in large amounts after distension of the cervix and uterus during labor, facilitating birth, and after stimulation of the nipples, facilitating breastfeeding.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Images |
Standard curve of the product EK00794E. |
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Specification |
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Quantity |
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