> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > General MMA/Methylmalonic Acid ELISA KitBrand |
Leading Biology | Catalog Number |
EK00780E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00780E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Methylmalonic Acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Methylmalonic Acid The ELISA analytical biochemical technique of the EK00780Ee kit is based on Methylmalonic Acid antibody-Methylmalonic Acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Methylmalonic Acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Methylmalonic Acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.099 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
MMA
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Function |
Methylmalonic acid (MMA) is a dicarboxylic acid that is a C-methylated derivative of malonate.
The coenzyme A linked form of methylmalonic acid, methylmalonyl-CoA, is converted into succinyl-CoA by methylmalonyl-CoA mutase, in a reaction that requires vitamin B12 as a cofactor. In this way, it enters the Krebs cycle, and is thus part of one of the anaplerotic reactions.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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