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Location: Home > Antigens, Antibodies, ELISA, Western Blo > Immunoassay Kits > ELISA Kit > General PYD/Pyridinoline ELISA Kit

General PYD/Pyridinoline ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00753E  In Stock 96T / $745

General PYD/Pyridinoline ELISA Kit

Brand

Leading Biology

Catalog Number

EK00753E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00753E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Pyridinoline) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pyridinoline The ELISA analytical biochemical technique of the EK00753Ee kit is based on Pyridinoline antibody-Pyridinoline antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pyridinoline antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Pyridinoline. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Pyridinoline is a fluorescent cross-linking compound of collagen fibers. Crosslinks in collagen and elastin are derived from lysyl and hydroxylysyl residues, a process catalyzed by lysyl oxidase. Fujimoto and colleagues first described the isolation and characterization of a fluorescent material in bovine achilles tendon collagen and termed it pyridinoline. It is reported to be present in collagen of bone and cartilage, but is absent in collagen of skin. It is not present in newly synthesized collagen and is formed from aldimine cross-links during maturation of collagen fibers.

Assay Type

Competitive

Detection Method

Spectrophotometrical

Reactivity

General

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

3.125nmol/L

Assay Range

6.25-400nmol/L

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

PYD

Function

Pyridinoline and deoxypyridinoline were found to be released into the blood during bone degradation and rapidly exereted in the urine. In a preliminary study, both these compounds were proposed as a marker for metastatic bone tumor in patients with prostate cancer.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Images

Standard curve of the product EK00753E.

Specification

Quantity

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