> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > General cholyglycine/cholyglycine ELISA KitBrand |
Leading Biology | Catalog Number |
EK00713E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00713e is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (cholyglycine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing cholyglycine The ELISA analytical biochemical technique of the EK00713Ee kit is based on cholyglycine antibody-cholyglycine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cholyglycine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, cholyglycine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Normal serum cholyglycine: 1.3 ± 0.8mg / L, range 0.4 to 2.98mg / L, and the low limit of the hepatitis diagnosis <3.18mg / L
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Assay Type |
Competitive
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Detection Method |
Spectrophotometrical
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Reactivity |
General
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.5 ng/mL
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Assay Range |
1.56-100 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
cholyglycine
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Function |
Serum cholyglycine (Cholyglycine, CG) is a combination of secondary bile acids with glycine conjugated bile acids, in liver cells, cholesterol, after its complex enzymatic reaction, turn into the primary bile acid. Cholic acid (CA) and chenodeoxycholic acid (the CD-CA). Bile acid steroid nucleus three hydroxyl groups (C3, C7, C12), the side chain hydroxyl at the end of the peptide bond and glycine combined with a molecular weight of 462u.
CG normal metabolic pathways of the intestine - liver circulation, CG synthesized by hepatocytes, bile canaliculi, bile duct discharged into the gall bladder, along with the bile into the duodenum to help digestion of food. 95% of bile acid reabsorption in the distal ileum, come back to the liver via the portal vein, and uptake by the liver cells to re-use. Exist in the form of protein binding in serum, the total amount of overflow into the systemic circulation is less than 1%. Under normal circumstances, bile acid content in the peripheral blood, leaving little room for normal adults, whether fasting or postprandial serum CG concentration stabilized at a low level.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
#N/A
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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