> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human PLA2G1B/Phospholipase A2 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00653E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00653E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Phospholipase A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Phospholipase A2 The ELISA analytical biochemical technique of the EK00653E kit is based on Phospholipase A2 antibody-Phospholipase A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Phospholipase A2 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Phospholipase A2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted secreted from pancreatic acinar cells in its inactive form.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.39 ng/mL
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Assay Range |
1.56-100 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
PLA2G1B
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MIM |
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UniProt ID |
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Function |
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Group IB phospholipase A2,Phosphatidylcholine 2-acylhydrolase 1B,PLA2,PLA2A,PPLA2
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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