> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human DEFB114/Beta-defensin 114 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00651E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00651E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Beta-defensin 114) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-defensin 114 The ELISA analytical biochemical technique of the EK00651E kit is based on Beta-defensin 114 antibody-Beta-defensin 114 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-defensin 114 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Beta-defensin 114. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
34 pg/mL
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Assay Range |
78-5000 pg/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
DEFB114
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MIM |
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UniProt ID |
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Function |
Has a salt-sensitive antimicrobial activity against Gram-negative bacteria, including E.coli, Gram-positive, including S.aureus, and fungi, including C.albicans. Binds to and neutralizes bacterial lipopolysaccharides (LPS), abolishing TNF production by macrophages challenged with LPS. Rescues the LPS-induced reduction of sperm motility in vitro and may protect from LPS-induced lethality.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Beta-defensin 14,DEFB-14,Defensin, beta 114,DEFB14
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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