> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human GDF5/Growth/differentiation factor 5 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00643E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00643E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Growth/differentiation factor 5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Growth/differentiation factor 5 The ELISA analytical biochemical technique of the EK00643E kit is based on Growth/differentiation factor 5 antibody-Growth/differentiation factor 5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Growth/differentiation factor 5 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Growth/differentiation factor 5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted Cell membrane
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.078 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
GDF5
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MIM |
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UniProt ID |
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Function |
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
GDF-5,Bone morphogenetic protein 14,BMP-14,Cartilage-derived morphogenetic protein 1,CDMP-1,Lipopolysaccharide-associated protein 4,LAP-4,Radotermin,BMP14,CDMP1
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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