> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human MITF/Microphthalmia-associated transcription factor ELISA KitBrand |
Leading Biology | Catalog Number |
EK00641E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00641E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Microphthalmia-associated transcription factor) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Microphthalmia-associated transcription factor The ELISA analytical biochemical technique of the EK00641E kit is based on Microphthalmia-associated transcription factor antibody-Microphthalmia-associated transcription factor antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Microphthalmia-associated transcription factor antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Microphthalmia-associated transcription factor. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Nucleus
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.35 ng/mL
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Assay Range |
0.625-40 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
MITF
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MIM |
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UniProt ID |
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Function |
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Class E basic helix-loop-helix protein 32,bHLHe32,BHLHE32
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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