> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human HIF1AN/Hypoxia-inducible factor 1-alpha inhibitor ELISA KitBrand |
Leading Biology | Catalog Number |
EK00618E |
Product Type |
ELISA Kit | Field of Research |
Epigenetics |
Product Overview |
EK00618E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Hypoxia-inducible factor 1-alpha inhibitor) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hypoxia-inducible factor 1-alpha inhibitor The ELISA analytical biochemical technique of the EK00618E kit is based on Hypoxia-inducible factor 1-alpha inhibitor antibody-Hypoxia-inducible factor 1-alpha inhibitor antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hypoxia-inducible factor 1-alpha inhibitor antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Hypoxia-inducible factor 1-alpha inhibitor. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Nucleus Cytoplasm Cytoplasm Perinuclear region Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.12 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
HIF1AN
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MIM |
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UniProt ID |
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Function |
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Factor inhibiting HIF-1,FIH-1,Hypoxia-inducible factor asparagine hydroxylase,FIH1
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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