> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human ASPRO/Asprosin ELISA KitBrand |
Leading Biology | Catalog Number |
EK00575E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00575E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Asprosin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Asprosin The ELISA analytical biochemical technique of the EK00575Eh kit is based on Asprosin antibody-Asprosin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Asprosin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Asprosin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Asprosin circulates at nanomolar levels, a single dose of recombinant asprosin retains biological activity and elevates blood glucose and insulin levels. Liver is the target organ for asprosin to recruit. Asprosin increases hepatocyte glucose release through activating the G protein-cAMP-PKA pathway.
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
null
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Asprosin
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UniProt ID |
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Function |
Circulating asprosin, a protein hormone, responds to low dietary glucose by triggering the release of liver glucose stores, and the reduction of asprosin protects against the hyperinsulinism associated with metabolic syndrome.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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