> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human HNRNPU/Heterogeneous nuclear ribonucleoprotein U ELISA KitBrand |
Leading Biology | Catalog Number |
EK00570E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00570E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Heterogeneous nuclear ribonucleoprotein U) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Heterogeneous nuclear ribonucleoprotein U The ELISA analytical biochemical technique of the EK00570E kit is based on Heterogeneous nuclear ribonucleoprotein U antibody-Heterogeneous nuclear ribonucleoprotein U antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Heterogeneous nuclear ribonucleoprotein U antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Heterogeneous nuclear ribonucleoprotein U. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Nucleus Nucleus matrix Chromosome Nucleus speckle Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Chromosome Centromere Kinetochore Cytoplasm Cytoskeleton Spindle Cytoplasm Cytoskeleton Spindle pole Midbody Cytoplasm Cell surface Cytoplasmic granule Localizes at inactive X chromosome (Xi) regions (PubMed:11003645, PubMed:14608463, PubMed:15563465). Localizes in the nucleus during interphase (PubMed:21242313). At metaphase, localizes with mitotic spindle microtubules (MTs) (PubMed:21242313). At anaphase, localizes in the mitotic spindle midzone (PubMed:21242313). Localizes in spindle MTs proximal to spindle poles in a TPX2- and AURKA-dependent manner (PubMed:21242313). The Ser-59 phosphorylated form localizes to centrosomes during prophase and metaphase, to mitotic spindles in anaphase and to the midbody during cytokinesis (PubMed:25986610). Colocalizes with SMARCA4 in the nucleus (By similarity). Colocalizes with CBX5 in the nucleus (PubMed:19617346). Colocalizes with NR3C1 in nuclear speckles (PubMed:9353307). Localized in cytoplasmic ribonucleoprotein (RNP) granules containing untranslated mRNAs (PubMed:17289661).
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.167 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
HNRNPU
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MIM |
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UniProt ID |
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Function |
(Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
hnRNP U,GRIP120,Nuclear p120 ribonucleoprotein,Scaffold-attachment factor A,SAF-A,p120,pp120,C1orf199,HNRPU,SAFA,U21.1
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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