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Location: Home > Antigens, Antibodies, ELISA, Western Blo > Immunoassay Kits > ELISA Kit > Human CANX/Calnexin ELISA Kit

Human CANX/Calnexin ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00562E  In Stock 96T / $645

Human CANX/Calnexin ELISA Kit

Brand

Leading Biology

Catalog Number

EK00562E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00562E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Calnexin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Calnexin The ELISA analytical biochemical technique of the EK00562E kit is based on Calnexin antibody-Calnexin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Calnexin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Calnexin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Endoplasmic reticulum membrane Single-pass type I membrane protein Endoplasmic reticulum Melanosome Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232).

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

0.17 ng/mL

Assay Range

0.312-20 ng/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

CANX

MIM

UniProt ID

Function

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Synonyms

IP90,Major histocompatibility complex class I antigen-binding protein p88,p90

Images

Standard curve of the product EK00562E.

Specification

Quantity

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