> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human THBS4/Thrombospondin-4 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00553E |
Product Type |
ELISA Kit | Field of Research |
Cardiovascular |
Product Overview |
EK00553E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Thrombospondin-4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Thrombospondin-4 The ELISA analytical biochemical technique of the EK00553E kit is based on Thrombospondin-4 antibody-Thrombospondin-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Thrombospondin-4 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Thrombospondin-4. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Endoplasmic reticulum Sarcoplasmic reticulum Secreted Secreted Extracellular space Secreted Extracellular space Extracellular matrix
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.045 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
THBS4
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MIM |
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UniProt ID |
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Function |
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
TSP4
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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