> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human IHH/Indian hedgehog protein ELISA KitBrand |
Leading Biology | Catalog Number |
EK00531E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00531E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Indian hedgehog protein) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Indian hedgehog protein The ELISA analytical biochemical technique of the EK00531E kit is based on Indian hedgehog protein antibody-Indian hedgehog protein antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Indian hedgehog protein antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Indian hedgehog protein. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cell membrane
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.098 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
IHH
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MIM |
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UniProt ID |
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Function |
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
IHH,HHG-2
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Images |
Standard curve of the product EK00531E. |
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Specification |
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Quantity |
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