> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human TP53/Cellular tumor antigen p53 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00473E |
Product Type |
ELISA Kit | Field of Research |
|
Product Overview |
EK00473E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cellular tumor antigen p53) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cellular tumor antigen p53 The ELISA analytical biochemical technique of the EK00473E kit is based on Cellular tumor antigen p53 antibody-Cellular tumor antigen p53 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cellular tumor antigen p53 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cellular tumor antigen p53. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
|
||
Cellular Localization |
Isoform 9 Cytoplasm
|
||
Assay Type |
Sandwich
|
||
Detection Method |
Spectrophotometrical
|
||
Reactivity |
Human
|
||
Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
|
||
Sensitivity |
0.089 ng/mL
|
||
Assay Range |
0.156-10 ng/ml
|
||
Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
|
||
Other Reagents Required |
Deionized or distilled water
|
||
Gene Name |
TP53
|
||
MIM |
|||
UniProt ID |
|||
Function |
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
|
||
Form |
ELISA Kits |
||
Storage & Stability |
Store at +4°C. Please refer to protocols.
|
||
Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
||
Synonyms |
Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53,P53
|
||
Images |
|
||
Specification |
|||
Quantity |
|
||
| Select | Brand | Catalog No. | Product Name | Pack Size | Type | Field of Research | Specification | Quantity | Price(USD) | |
| 1 | Leading Biology | EK00487E | Human DCN/Decorin ELISA Kit | 96T | ELISA Kit |
|
$645.00 | Add Ask | ||
| 2 | Leading Biology | EK00726E | General HS/Heparan sulfate ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask | ||
| 3 | Leading Biology | EK00038E | General E2/Estradiol ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
| 4 | Leading Biology | EK0003L | Exendin-4 (Exenatide) ADA | 96T | ELISA Kit |
|
$895.00 | Add Ask | ||
| 5 | Leading Biology | EK00003E | General Testosterone/Testosterone ELISA Kit | 96T | ELISA Kit |
|
$545.00 | Add Ask | ||
| 6 | Leading Biology | EK00700E | General DHEA/Dehydroepiandrosterone ELISA Kit | 96T | ELISA Kit |
|
$745.00 | Add Ask |
Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
You have 0 item in your cart
You have 0 item in your inquiry list
