> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Rat Nos3/Nitric oxide synthase, endothelial ELISA KitBrand |
Leading Biology | Catalog Number |
EK00433E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00433E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Nitric oxide synthase, endothelial) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nitric oxide synthase, endothelial The ELISA analytical biochemical technique of the EK00433E kit is based on Nitric oxide synthase, endothelial antibody-Nitric oxide synthase, endothelial antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nitric oxide synthase, endothelial antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Nitric oxide synthase, endothelial. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Membrane Caveola Cytoplasm Cytoskeleton Golgi apparatus Cell membrane Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Rat
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.078 ng/mL
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Assay Range |
0.156-10 ng/ml
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Nos3
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UniProt ID |
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Function |
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Constitutive NOS,cNOS,EC-NOS,Endothelial NOS,eNOS,NOS type III,NOSIII
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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