> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Rat Mmp2/72 kDa type IV collagenase ELISA KitBrand |
Leading Biology | Catalog Number |
EK00412E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00412E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (72 kDa type IV collagenase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 72 kDa type IV collagenase The ELISA analytical biochemical technique of the EK00412E kit is based on 72 kDa type IV collagenase antibody-72 kDa type IV collagenase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 72 kDa type IV collagenase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 72 kDa type IV collagenase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted Extracellular space Extracellular matrix Membrane Nucleus Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Rat
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.168 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Mmp2
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UniProt ID |
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Function |
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta3 on the surface of blood vessels.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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