> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Mouse Nos1/Nitric oxide synthase, brain ELISA KitBrand |
Leading Biology | Catalog Number |
EK00330E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00330E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Nitric oxide synthase, brain) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nitric oxide synthase, brain The ELISA analytical biochemical technique of the EK00330E kit is based on Nitric oxide synthase, brain antibody-Nitric oxide synthase, brain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nitric oxide synthase, brain antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Nitric oxide synthase, brain. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Cell membrane Sarcolemma Peripheral membrane protein Cell projection Dendritic spine In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Mouse
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.156 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Nos1
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UniProt ID |
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Function |
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Constitutive NOS,NC-NOS,NOS type I,Neuronal NOS,N-NOS,Peptidyl-cysteine S-nitrosylase NOS1,bNOS
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Images |
Standard curve of the product EK00330E. |
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Specification |
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Quantity |
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