> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Mouse Mmp2/72 kDa type IV collagenase ELISA KitBrand |
Leading Biology | Catalog Number |
EK00283E |
Product Type |
ELISA Kit | Field of Research |
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Product Overview |
EK00283E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (72 kDa type IV collagenase) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 72 kDa type IV collagenase The ELISA analytical biochemical technique of the EK00283E kit is based on 72 kDa type IV collagenase antibody-72 kDa type IV collagenase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 72 kDa type IV collagenase antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 72 kDa type IV collagenase. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Isoform 2 Cytoplasm Mitochondrion
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Mouse
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.167 ng/mL
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Assay Range |
0.312-20 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
Mmp2
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UniProt ID |
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Function |
Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
72 kDa gelatinase,Gelatinase A,Matrix metalloproteinase-2,MMP-2
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Images |
Standard curve of the product EK00283E. |
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Specification |
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Quantity |
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