> Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human TIMP1/Metalloproteinase inhibitor 1 ELISA KitBrand |
Leading Biology | Catalog Number |
EK00193E |
Product Type |
ELISA Kit | Field of Research |
Cardiovascular |
Product Overview |
EK00193E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Metalloproteinase inhibitor 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Metalloproteinase inhibitor 1 The ELISA analytical biochemical technique of the EK00193E kit is based on Metalloproteinase inhibitor 1 antibody-Metalloproteinase inhibitor 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Metalloproteinase inhibitor 1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Metalloproteinase inhibitor 1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
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Cellular Localization |
Secreted
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Assay Type |
Sandwich
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Detection Method |
Spectrophotometrical
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Reactivity |
Human
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Sample Type |
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
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Sensitivity |
0.078 ng/mL
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Assay Range |
0.156-10 ng/mL
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Kit Components |
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
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Other Reagents Required |
Deionized or distilled water
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Gene Name |
TIMP1
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MIM |
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UniProt ID |
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Function |
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.
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Form |
ELISA Kits |
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Storage & Stability |
Store at +4°C. Please refer to protocols.
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Applications |
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Synonyms |
Erythroid-potentiating activity,EPA,Fibroblast collagenase inhibitor,Collagenase inhibitor,Tissue inhibitor of metalloproteinases 1,TIMP-1,CLGI,TIMP
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Images |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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