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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human TGFB2/Transforming growth factor beta-2 ELISA Kit

Human TGFB2/Transforming growth factor beta-2 ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00173E 96T / $545

Human TGFB2/Transforming growth factor beta-2 ELISA Kit

Brand

Leading Biology

Catalog Number

EK00173E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00173E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Transforming growth factor beta-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Transforming growth factor beta-2 The ELISA analytical biochemical technique of the EK00173E kit is based on Transforming growth factor beta-2 antibody-Transforming growth factor beta-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Transforming growth factor beta-2 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Transforming growth factor beta-2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Transforming growth factor beta-2 Secreted

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

7.8 pg/mL

Assay Range

15.6-1000 pg/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

TGFB2

MIM

UniProt ID

Function

Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development (PubMed:22772371, PubMed:22772368). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Synonyms

Cetermin,Glioblastoma-derived T-cell suppressor factor,G-TSF

Images

Standard curve of the product EK00173E.

Specification

Quantity

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