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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human IL12B/Interleukin-12 subunit beta ELISA Kit

Human IL12B/Interleukin-12 subunit beta ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00050E 96T / $545

Human IL12B/Interleukin-12 subunit beta ELISA Kit

Brand

Leading Biology

Catalog Number

EK00050E

Product Type

ELISA Kit

Field of Research

Product Overview

EK00050E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Interleukin-12 subunit beta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Interleukin-12 subunit beta The ELISA analytical biochemical technique of the EK00050E kit is based on Interleukin-12 subunit beta antibody-Interleukin-12 subunit beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Interleukin-12 subunit beta antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Interleukin-12 subunit beta. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Secreted

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

12 pg/mL

Assay Range

31.2-2000 pg/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

IL12B

MIM

UniProt ID

Function

Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Synonyms

IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40,NK cell stimulatory factor chain 2,NKSF2,NKSF2

Images

Standard curve of the product EK00050E.

Specification

Quantity

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