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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Immunoassay Kits > ELISA Kit > Human IgG/Immunoglobulin G ELISA Kit

Human IgG/Immunoglobulin G ELISA Kit

Catalog # Availability Size / Price Inquiry
EK00010E 96T / $545

Human IgG/Immunoglobulin G ELISA Kit

Brand

Leading Biology

Catalog Number

EK00010E

Product Type

ELISA Kit

Field of Research

Immunology

Product Overview

EK00010E is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin G) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin G The ELISA analytical biochemical technique of the EK00010E kit is based on Immunoglobulin G antibody-Immunoglobulin G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin G antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin G. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Cellular Localization

Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains — two heavy chains γ and two light chains. Each IgG has two antigen binding sites. Other immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells.

Assay Type

Sandwich

Detection Method

Spectrophotometrical

Reactivity

Human

Sample Type

This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Sensitivity

29.2 pg/mL

Assay Range

78-5000 pg/mL

Kit Components

Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

Other Reagents Required

Deionized or distilled water

Gene Name

IgG

Function

IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response.

Form

ELISA Kits

Storage & Stability

Store at +4°C. Please refer to protocols.

Applications

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Images

Standard curve of the product EK00010E.

Specification

Quantity

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