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Custom Monospecific Antibody Production

Introduction Custom Monospecific Antibody Production is our most popular package. It can serve as a cost-effective alternative to monoclonal antibody production for many applications. By immunizing with a peptide sequence and then affinity purifying the serum against peptide sequence, it is possible to isolate highly specific antibodies that approach the specificity of monoclonal antibodies but provide the superior affinity of polyclonal antibodies. Details Protein Sequence Analysis lConjugation of 3-5 mg of the peptide to KLH l2 Rabbits u4 Immunizations per animal uProvides approximately 160-200 ml of anti-sera lAffinity Purification of 25 ml of Serum from 3rd Production Bleed lHandling / Shipment Preparation Service Process Details Cost per each additional peptide residue over the 15 residues included with the package ...

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Anti-Membrane Protein Antibody Discovery

Anti-Membrane Protein Antibody Discovery Using Hybridoma Leading Biology has extensive experience in hybridoma technology. Our mature hybridoma platform offers both mouse and rat animal models to raise anti-GPCR antibodies. Vigorous immune response and high cell fusion efficacy can be guaranteed, followed by comprehensive screening of large candidate pools. Our team is specialized in various high throughput screening strategies against recombinant proteins, reconstituted formats, living cells, etc. Of note, we have established a novel transgenic mouse strain that can produce fully human anti-GPCR antibodies, which can greatly facilitate therapeutic/diagnostic antibody discovery. Anti-Membrane Protein Antibody Discovery by Phage Display Technology Leading Biology offers one-step phage display library construction and screening service. Equipped with this world-leading phage display platform, we have rich experience in conducting versatile bio-panning strategies, including in-solution panning, solid-phase panning, cell-based panning, in vivo panning, etc. ...

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Phage Display Library Construction

Introduction Leading Biology is pleased to offer customized library construction services under a variety of phage display systems. Our scientists have extensive experience in library construction and are professional in generating different types of phage display libraries, including scaffold libraries, peptide libraries, antibody libraries, cDNA libraries and others. Phage Display Library Types Antibody library construction service Scaffold library construction service Peptide library construction service cDNA library construction service Antibody Library (Immune, Naive, Semi-synthetic, Synthetic) Leading Biology can display the interest antibody in either scFv or Fab format to construct a high-quality antibody library. Due to the smaller size, scFv libraries are genetically more stable than Fab libraries, while Fab libraries lack the tendency to form higher molecular weight species, such as dimers and trimers, which can simplify the selection and characterization. Moreover, both human plasma cells and splenocytes from naïve or immune anima...

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Phage Display & Antibody Library Services

Phage Display Service M13 single strand filamentous phage display system is the most widely used library display system. The phage display library is formed by fusing the protein, antibody or polypeptide with phage pIII protein to participate in phage package and display on the phage surface. Using phage display library to make 3-5 rounds of affinity screening for specific target molecules, phage that specifically binds to the target molecule could be enriched to obtain corresponding DNA sequence information. Our current phage display technology service platform covers frequently-used library types such as immune antibody library and natural antibody library. We strive for fulfilling individual customer's needs by customizing a variety of library and screening services to fit the individual preference with flexibility and efficiency. Phage Display Service Profile Leading Biology platform system mainly includes pComb3 phage display system and pCANTAB5e phage display system. pComb3 Phage Display Technology ...

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Anti-Ion Channel Antibody Production Service

Introduction Ion channels are also a class of important membrane proteins. The normal physiological activities of some cells and the pathological processes of some diseases are achieved through the conformation of modified membrane channels, so ion channel proteins also an important target for some drugs. At present, based on the small molecule through membrane channel, people have developed several drugs that act on specific cell ion channels, such as Aspirin, a drug which we all know, is a commonly used proton pump inhibitor. Although based on the specificity and efficiency of antibody binding, people have developed some antibody drugs targeted on ion channel proteins, but the development of the antibody drug is not easy. A main reason is that the expression and purification of ion channel protein which used as an immunogen still facing many problems, it is hard to prepare enough amount of proteins to activate the immune response, in addition to immunization, the screening also needs a large number of target antigens, this brought enormous pressure on the preparation of antigens. In order to solve the problems above, Leading Biology has es...

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Anti-GPCR Antibody Production

Introduction G Protein-Coupled Receptor (GPCR) family is a class of 7 transmembrane proteins, it is a target for many diseases. For some drugs which can not target the GPCR have been proved to have pharmacological activity under the guidance of some GPCR target molecules. With the development of immune technology, especially the improvement of the preparation and purification of GPCR protein, more and more drugs are considering using antibodies to target GPCR. The most notable one among these antibodies is the heavy chain antibody (hcAbs) which from camel (Dromedary), alpaca and other Camelidae animals. The antibody which targets the binding domain of hcAb antigen (also known as single domain antibody), at the same time has a low molecular weight (low steric blockade and low expression cost) and monoclonal characteristics (high affinity and specificity), is the ideal guidance molecule for GPCR coupling drugs. Fig.1 The size of different types of antibody fragments and their common hosts Advantage With the breakthroughs in structural biology and biochemistry technology, more and more GPCR can be synthesized in...

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Hybridoma Preparation and Anti-membrane Protein mAbs Product

Introduction Although using the traditional antibody preparation method to prepare antibodies against membrane protein facing lots of difficulties, considering the overall time and technical difficulty, monoclonal antibody preparation for some simple structure membrane proteins is still an economical and effective way. Service Package Service Items Time(weeks) Peptide Immunization Recombination Protein Immunization Peptide design & synthesis Peptide design -2 Free, Optional Peptide synthesis √ √ Conjugation √ ...

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Membrane Protein Antibody Production by Whole Cell APC

Introduction At the initial of mammalian immune process, antigens are not directly processed by Th cells, but processed and presented by the antigen-presenting cells (APC). If we can produce cells that express target antigens, these antigens will be expressed on the cell surface, this kind of cell can be used to immunize animals as antigens. The advantages of this method are: in situ expression of transmembrane protein in eukaryotic system ensures its complete post-translational modification process, meanwhile, the natural structural characteristics are well preserved by using the cell membrane as they assemble and display surface. However, it also has many disadvantages: since the cell is too large compared to the target antigen, and the surface also contains some modificated non-target antigens, so as an antigen, although the immunogenicity can be guaranteed, the validity and specificity are not. The solution of this problem including high expression of target antigen and a large number of screening to identify and exclude antibodies which recognize invalid antigens. Service Package Service I...

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Membrane Protein Antibodies Service

Introduction Among the numerous genes in the genome, there are 20%-30% gene encodes the membrane protein. These membrane proteins are involved in almost every intracellular activities in life, including ligand-receptor binding, signal transduction, molecular transport, intercellular recognition and enzyme catalysis. Since the membrane proteins participate in such a large number of physiological activities, it's an important target for drug development and clinical research. As an important reagent in experimental research, the antibodies which against these membrane proteins are essential, however, there are many problems by using traditional methods to prepare these antibodies, including the low expression rate, hard extraction and purification, heterologous gene encoding, mis-folding, incomplete post-translational processing and conformational differences, etc. Leading Biology has established custom membrane protein and membrane protein antibody production platforms for antibody discovery. Membrane proteins, actually, are a kind of proteins acting as ion channels, receptors and transporters, which enable the cells to transport e...

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Anti-autologous Antigen Antibody Production Service

Introduction The preparation of anti-autologous antigen antibody refers to the use of normal proteins and small molecules which exists in the normal immune animals, or proteins and small molecules from other species which have high sequence conservation or structural consistency with natural immune animals as an antigen or hapten to immune these animals themselves to prepare the corresponding antibodies. Since the immunogens exist in natural immune animals, the animal immune system is hard to start the cleaning reaction (immune reaction) as a reaction to exogenous substances, this reaction is regulated by the self-protective mechanism, because once these foreign antigens were considered as an immunogen, it will cause the immune system to attack the normal proteins which have similar structures, induce autoimmune diseases. Therefore, the immunogenicity of these self-antigens are often very low, if not consider replacing the host species, it is difficult to prepare, but sometimes, due to the limitation of experimental conditions, we cannot make this choice, so the preparation of anti-autologous antigen antibody is very difficult. Rely on the experience ...

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