Introduction

Leading Biology is pleased to offer customized library construction services under a variety of phage display systems. Our scientists have extensive experience in library construction and are professional in generating different types of phage display libraries, including scaffold libraries, peptide libraries, antibody libraries, cDNA libraries and others.

Phage Display Library Types

• Antibody library construction service
• Scaffold library construction service
• Peptide library construction service
• cDNA library construction service

Antibody Library

(Immune, Naive, Semi-synthetic, Synthetic)

Leading Biology can display the interest antibody in either scFv or Fab format to construct a high-quality antibody library.

Due to the smaller size, scFv libraries are genetically more stable than Fab libraries, while Fab libraries lack the tendency to form higher molecular weight species, such as dimers and trimers, which can simplify the selection and characterization. Moreover, both human plasma cells and splenocytes from naïve or immune animals can be accepted as repertoire source for our antibody library construction service. In addition, Leading Biology is able to generate fully or semi-synthetic antibody libraries as well.

Scaffold Library

Leading Biology is able to generate high-quality phage display libraries for a comprehensive list of scaffolds. Our scientists devote to offer customized service to design specific scaffold based on clients’ demands. In addition, to achieve 100% precise mutant and over 1010 diversity, both trimer codon technology and NNK method are used in the library construction resulting in an increased randomicity, which enables the library to be more suitable for sorting and isolating the high-affinity protein or peptide targets.

Peptide Library (Naive, Semi-synthetic)

To form a peptide library, Leading Biology can generate constrained structures with target-adapted cross-links for both naïve and semi-synthetic peptides. This structure contributes to maintaining or increasing a series of properties of peptides, such as stability, permeability, binding affinity and resistance to protease activity. Furthermore, for peptide libraries with inserts longer than 8-mer (and up to 30-mer), three nucleotide doping strategies (NNK, Split-mix-split synthesis, and Trimer codon) can be adapted to maximize the amino acid sequences encoded by the inserted DNA sequences.

cDNA Library

According to our advanced phage display platform, Leading Biology is capable of displaying entire cDNA products on the surface of an appropriate phage system. Compared with conventional cDNA libraries, our high-quality libraries can achieve higher capacity, density, and correct orientation, which further contribute to the investigation of a lot of protein candidates. Moreover, there are various types of cDNA libraries, including standard library, full-length library, normalized library and subtractive library, which can be tailored to match our clients’ demands.

Diversity of Semi-synthetic/Synthetic Libraries

In general, Leading Biology employs three main approaches to design semi-synthetic or fully synthetic libraries with appropriate diversity. Our scientists normally generate libraries with a diversity of 108, while we are also able to generate libraries over 1010 or follow our clients’ specific requirements.

Specific requirements as follows:

·      Hard randomization: complete diversity is introduced by using degenerate codons encoding all twenty natural amino acids.

·      Tailored randomization: incomplete or biased diversity is introduced by using degenerate codons encoding a subset of natural amino acids.

·      Diversification: degenerate codons are synthesized using trimer phosphoramidites corresponding to 20 natural amino acids, which exclude stop codons.

Why Leading Biology?

We offer the one-stop phage display service:

·      Immune, naive or synthetic library construction

·      Pre-made library screening

·      Monoclonal detection

·      Antibody expression

·      Custom biopanning strategies

·      QC: to ensure the success of your projects, we perform documentation QC for all assays.

Our advantages

·      World-leading sequencing capacity: use state-of-the-art technologies, such as Timer Condon technology, nanopore platform, etc with library capacity from 10⁸ to over 10¹³ ;

·      Various phage display systems: provide the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands.

·       High affinity: from 10^-7 to 10^-9

·      Custom biopanning strategies: The experienced technical team will work together to customize our products so as to meet our customers’ different needs. Leading Biology also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naive targets. Our Naive human antibody library panning includes: Pre-made yeast display naive human antibody library & Pre-made phage display naive human Fab library.

·      Rich experience: Our technicians refine every detail of our processing to enable the efficiency and high-quality. With extensive experience in phage display, we have provided satisfying service to our global researchers.

Contact US

We are proud to be able to support global scientists by providing high-quality service in a rapid time-frame. Reach out to us and we will get back to you within 24 hours.

Get a free quote

Email: info@leadingbiology.com

Phone: 1-661-524-0262 (USA)