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Location: Home > Antigen, Antibodies, ELISA, Western Blot > NADP/NADPH Assay Kit

NADP/NADPH Assay Kit

Catalog # Availability Size / Price Inquiry
EP0001 100T / $875

NADP/NADPH Assay Kit

Brand

Leading Biology

Catalog Number

EP0001

Product Type

Antigen, Antibodies, ELISA, Western Blot

Field of Research

Assay Type

Quantitative

Sample Type

Tissue Extracts, Cell Lysate

Storage & Stability

Store at -20°C. Please refer to protocols.

Product Description

NADP/NADPH Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue.


The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. The assay does not recognize NAD+/NADH. There is no need to purify NADP/NADPH from the sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using a plate reader at OD450nm.


NADP / NADPH assay protocol summary:
- extract samples from cells /  tissues with extraction buffer and deproteinize with spin column
- for NADPH measurement, heat samples to 60ºC for 30 min to decompose NAD+, cool on ice (this step not necessary for measurement of total NADP+/NADPH)
- add samples and standards to wells
- add reaction mix and incubate for 5 min at room temp to convert NADP to NADPH
- add NADPH developer and incubate for 1-4 hrs while reaction cycles
- analyze with microplate reader multiple times during the 1-4 hr incubation
- reaction can be stopped with stop solution


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