> Antigen, Antibodies, ELISA, Western Blot > Anti - MOG(35 - 55) IgG Quantitative ELISA Kit (mouse/rat)Brand |
Leading Biology | Catalog Number |
PE0008 |
Product Type |
Antigen, Antibodies, ELISA, Western Blot | Field of Research |
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Product Overview |
This kit is optimized to detect mouse/rat anti-MOG (35-55) IgG. Wells are pre-coated with MOG (35-55)
peptide and pre-blocked with BSA. The amount of anti-MOG (35-55) IgG in serum or cerebrospinal
fluid is quantified using ELISA. Ample materials and reagents are provided to perform 96 assays.
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Sample Type |
Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assay
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Sensitivity |
Detects as low as 100 pg anti-MOG (35-55) IgG
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Assay Range |
8-500 ng antibody/ml serum (depending on colorimetric developing time)
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Kit Components |
MOG (35-55) coated and BSA blocked 8-well strips
Mouse anti-MOG (35-55) IgG standard
1X Sample Dilution Buffer
10X Wash Buffer
TMB color substrate solution
Stop Solution
Secondary antibody, Goat anti-Mouse IgG-HRP
Secondary antibody, Goat anti-Rat IgG-HRP
Rat anti-MOG (35-55) IgG standard
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Other Reagents Required |
Microplate reader: Capable of reading absorbance at 450 nm
Rocking platform or shaker
Strip ejector (to eject strips for future assay if not all strips are used in one experiment)
Computer software: Capable of plotting Four Parameter Logistic Curve Fit (4-PL) (optional)
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Storage & Stability |
Kit is shipped on blue ice. Store all kit components at 2-8℃ for up to 12 months.
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Product Description |
MOG peptide fragment (35-55) induces autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination. Autoantibody response to MOG (35-55) is present in multiple sclerosis (MS) patients and MOG (35-55)-induced experimental autoimmune encephalomyelitis (EAE) mice.
Anti-MOG (35-55) IgG Quantitative ELISA Kit (mouse/rat) provides a convenient, quantitative assay for detecting anti-MOG (35-55) IgG. This colorimetric assay, useful for determining the amount of anti-MOG (35-55) IgG present, can help provide information on the role anti-MOG (35-55) IgG plays in the development of EAE, an in vivo animal model for MS pathogenesis. The assays are performed in a convenient 96-well (strips format). The kit contains: • Pre-coated and pre-blocked 96-well strip plate • Standard for calibration • A detailed protocol Kit Size: 96 assays |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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