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Location: Home > Antigen, Antibodies, ELISA, Western Blot > Secondary Antibody > Anti-M13 Bacteriophage polyclonal antibody(HRP)

Anti-M13 Bacteriophage polyclonal antibody(HRP)

AH0013R1
Catalog # Availability Size / Price Inquiry
AH0013R1 100μL / $395

Anti-M13 Bacteriophage polyclonal antibody(HRP)

Brand

Leading Biology

Catalog Number

AH0013R1

Product Type

Secondary Antibody

Field of Research

Product Overview

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic nor temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.

Host

Rabbit

Immunogen

M13 Bacteriophage

Conjugation

HRP

Form

Liquid

Purity

Purification: Protein A

Storage & Stability

stored at -20°C to -80°C , Avoid repeated freeze-thaw cycle

Storage Buffer

PBS (pH=7.40)

Dilution

ELISA: 1:3000~1:5000

Product Description

Antibody notes

Can be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

References

Messing, J . et al., (1993). Methods in Molecular Biology. 23. pp. 9–22. Phage Display: A Laboratory Manual Sitohy, M. et al., (2006). J Agric Food Chem. 54 (11): 3800-6. Phipps ML et al. , (2016). PLoS One11:e0160940

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