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ALDOC Antibody (C-term)

APG01804G Product Datasheet Protocol

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APG01804G		100 μl / $495

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Leading Biology Inc.

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ALDOC Antibody (C-term)

Brand	Leading Biology	Catalog Number	APG01804G
Product Type	Monoclonal Antibodies	Field of Research
Product Overview	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ALDOC antibody (C-term).
Molecular Weight	H=39;M=39;R=39 KDa
Host	Mouse
Species Reactivity	Human, Mouse, Rat
Immunogen	1-364 aa
Target	Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
Symbol	ALDC
GeneID	230
UniProt ID	P09972
Form	Liquid. In PBS containing 0.02% sodium azide.
Storage & Stability	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Applications	WB
Dilution	FC~~1:25 WB~~1:2000
Synonyms	Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
Images	Overlay histogram showing HL-60 cells stained with APG01804G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APG01804G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG3 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-ALDOC Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: rat brain lysate Lane 4: mouse brain lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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