> Antigen, Antibodies, ELISA, Western Blot > Primary Antibody > Monoclonal Antibodies > Oct4 AntibodyBrand |
Leading Biology | Catalog Number |
APR08864G |
Product Type |
Monoclonal Antibodies | Field of Research |
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Product Overview |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Oct4 antibody.
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Molecular Weight |
45kDa
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Cellular Localization |
Antigen Cellular Localization:
Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern
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Host |
Mouse
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Species Reactivity |
Human
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Clone |
7E7
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Isotype |
IgG1
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Symbol |
OCT3, OCT4, OTF3
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GeneID |
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UniProt ID |
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Function |
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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Form |
Liquid. In PBS containing 0.02% sodium azide. |
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Storage & Stability |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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Applications |
WB, FC, ICC, E
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Dilution |
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
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Synonyms |
POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3
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Images |
Figure 1: Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.
Figure 2: Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb (green) and negative control (purple). |
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Specification |
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Quantity |
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Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
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